Regulation of motility of Salmonella enterica serovar Typhi by QseBC

VernacularTitle:QseBC双组分调控系统对伤寒沙门菌动力的调节作用
Author: Ying JI ; Bin NI ; Yiquan ZHANG ; Ruifu YANG ; Xinxiang HUANG
Publication Type:Journal Article
Keywords: Salmonella enterica serovar Typhi; QseBC; flhD; motility
From: Military Medical Sciences 2015;(5):357-359,367
CountryChina
Language:Chinese
Abstract: Objective To study the impact of QseBC on the motility of Salmonella enterica serovar Typhi ( S.Typhi ) . Methods The motility of wild-type ( WT) and null mutants (ΔqseB and ΔqseC) at mid-log phase was investigated by swimming assay.Quantitative RT-PCR was carried out to calculate the transcriptional variation of flhD and qseB among WT,ΔqseB andΔqseC.QseB overexpressing strain was constructed to compare its motility and flhD expression with the wild-type control.Results The result of motility assay showed that the motility of ΔqseB was similar to that of the WT strain , while the motility of ΔqseC was much lower than that of WT .qRT-PCR revealed that compared with WT , the expression of flhD was significantly decreased in ΔqseC while the expression of qseB was increased considerably .The motility of QseB overex-pressing strain was lower .Conclusion The expression of flhD may be regulated by QseBC which has an effect on the motil-ity of S.typhi, and the overexpression of QseB may inhibit the motility .