Diagnostic value of IRG1 in Mycobacterium tuberculosis infection

VernacularTitle:IRG1 mRNA定量检测对结核感染诊断的价值
Author: Zhihong CAO ; Yan CAO ; Xiaoxing CHENG
Publication Type:Journal Article
Keywords: Tuberculosis; Chemokines; IRG1; LTBI
From: Chinese Journal of Immunology 2015;(5):667-669,673
CountryChina
Language:Chinese
Abstract: Objective:To compare the mRNA expression of IRG1in PBMCs from patients with TB, individual with latent infection and healthy controls, and investigate its diagnostic value for Mycobacterium tuberculosis infection.Methods: The mRNA expression of IRG1 from PBMCs stimulated with Mtb-specific antigens was quantitatively detected by quantitative real-time PCR ( qPCR).Receiver-operating-characteristic ( ROC) curves were used to determine the cutoff points yielding the highest specificity and sensitivity,and discriminative ability was evaluated by the area under the ROC curve.Results: The mRNA expression of IRG1 in healthy controls was significantly lower than that in patients with tuberculosis and LTBI ( P<0.05 ).The AUC for using IRG1 mRNA levels (>0.01536 ) to identify Mycobacterium tuberculosis infection was 0.91, with 76.47% sensitivity, 96.30% specificity, LR 20.65,and 85.2% of cases correctly classified.Conclusion: IRG1 may be used as a biomarker for diagnosing Mycobacterium tuberculosis infection.