Enterovirus 71 molecular epidemiology research in Nantong,2014
10.3969/j.issn.1673-4130.2015.10.014
- VernacularTitle:南通地区2014年肠道病毒71型的分子流行病学研究
- Author:
Hongping ZHANG
;
Min ZHOU
;
Renfei LU
;
Xuemei LI
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
genetic traits;
molecular epidemiology
- From:
International Journal of Laboratory Medicine
2015;(10):1354-1355,1357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze enterovirus 71 (EV71) molecular epidemiology characteristics in Nantong region of Jiangsu , 2014 .Methods The pharyngeal swab samples were selected from 52 children with hand ,foot and mouth disease .After extracting the virus nucleic acid ,the EV71 VP1 gene of the virus were amplified by RT‐PCR amplification .The phylogenetic tree was con‐structed between isolated and EV71 reference strains from other parts of the country .Results 12 strains EV71 VP1 gene were iso‐lated from 52 cases of clinical specimens .The nucleotide and amino acid homology of 12 strains EV71 VP1 gene was 93 .4% -99 .1% ,the phylogenetic tree analysis showed that 12 strains of VP1 gene all belong to C4 genotype C4a subgenotypes .Conclusion The isolated strains in Nantong in 2014 are all the EV71 C4a subgenotypes of C4 genotype ,the same with the most of isolates in re‐cent years .
