Analysis of Mmultiple Viral Antigens in Pediatric Patients Detection Result by Direct Immunofluorescence Method
10.16466/j.issn1005-5509.2015.05.006
- VernacularTitle:直接免疫荧光法分析儿童呼吸道病毒的分布
- Author:
Hui FENG
;
Yonglin LIU
;
Yimin CHEN
- Publication Type:Journal Article
- Keywords:
direct immunofluorescence;
espiratory virus;
antigen;
respiratory infectious disease;
children
- From:
Journal of Zhejiang Chinese Medical University
2015;(5):348-350,351
- CountryChina
- Language:Chinese
-
Abstract:
Purpose] To detect respiratory virus antigen by direct immunofluorescence method and provide evidence for early diagnosis of children with viral infection of the respiratory tract disease.[Methods] Select 398 cases of respiratory tract infection in hospitalized children, pharynx in exfoliated cells in respiratory syncytial virus by direct immunofluorescence(RSV), adenovirus(ADV), influenza virus type A(IFVA),influenza virus type B(IFVB), parainfluenza virus type 1(PIV1), parainfluenza virus type 2(PIV2) and parainflue-Nza virus type 3(PIV3) of 7 common viral antigens were detected, and carried on statistics analysis.[Results] In 398 cases of children with respiratory tract infection, 91 cases were positive, the positive rate was 22.86%. The highest for respiratory syncytial virus in 37 cases(40.66%) followed by parainfluenza virus type 3 in 19 cases(20.88%), influenza virus type B in 14 cases(15.38%); The positive rates of acute tonsillitis, capillary bronchitis, pneumonia, acute bronchitis and upper respiratory were 46.67%(14/30),40.00%(20/50), 26.67%(40/150), 18.42%(7/38), 7.69%(10/130) respectively; With the growth of age, the respiratory tract virus infection rate gradually decreased(P<0.05), one year old the viral infection rate was 76.92%;it occurred in the winter season of respiratory tract infection in children .[Conclusion] The main virus in children with acute respiratory infection in the local area is RSV. Virus infection decreases with the increase of age, treatment should be careful in the use of antibiotics.
