Establishment of evaluation methods for the performance of flow cytometer
10.3969/j.issn.1673-4130.2015.10.020
- VernacularTitle:流式细胞仪性能评价方法的建立
- Author:
Xiaolin WANG
;
Ang LI
;
Shuo YANG
- Publication Type:Journal Article
- Keywords:
flow cytometry;
performance evaluation;
BriCyte E6
- From:
International Journal of Laboratory Medicine
2015;(10):1366-1367,1369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Establish evaluation methods for the performance of flow cytometer .Methods Referring to the indus‐try standard YY/T0588‐2005 Flow Cytometry ,evaluating methods for the performance of BriCyte E6 flow cytometry was estab‐lished ,such as fluorescence sensitivity ,fluorescence linearity ,forward scatter sensitivity ,instrument resolution ,forward scatter/side scattering resolution ,DNA content linearity ,carry‐over rate ,accuracy of the cell surface marker ,reproducibility of the cell surface marker and instrument stability .Results The performance of BriCyte E6 met the requirements of industry standard .Conclusion The evaluation methods for the performance parameters could be reliable and could be used for the performance evaluation of flow cytometer .
