Effects of PDS-C on immunoregulation in mice with aplastic anemia
10.3969/j.issn.1001-1978.2015.06.011
- VernacularTitle:人参二醇组皂苷提取物对再生障碍性贫血小鼠免疫调节作用的研究
- Author:
Zhiyin ZHENG
;
Liming YIN
;
Haifeng ZHUANG
;
Zhi CHENG
;
Yannan ZHAO
;
Xiaoling YU
;
Ruilan GAO
- Publication Type:Journal Article
- Keywords:
panaxdiol saponins component;
aplastic a-nemia;
immune regulation;
T cell subsets;
mice;
ex-perimental studies
- From:
Chinese Pharmacological Bulletin
2015;(6):790-794,795
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effects of panaxdiol saponins component ( PDS-C) extracted and isolated
from Chinese ginseng herb as new Chinese patent med-icine on the promotion of hematopoiesis and the regula-tion of the immune system in treating mice models with aplastic anemia ( AA ) . Methods For preparation of immune mediated AA models, BALB/c mice were ex-posed to sublethal doses of 5. 0 Gy γ radiation, fol-lowed by transplanted lymphocytes from DBA/2 donor mice. The mice models were divided into six groups in-cluding normal control, AA model, PDS-C treated groups with lower, medium and higher dosages, cy-closporine ( CsA) as positive drug control. Both PDS-C and CsA were administered by gastrogavage for 15 days. The peripheral blood cells counts and bone mar-row pathological examination were tested, the percenta-ges of Th1/Th2/Treg cells from spleen were measured, the protein expression levels of T-bet, GATA-3 and FOXP3 transcription factors in spleen cells were detec-ted. Results Curative effect of PDS-C on treating AA
mice was satisfactory. The peripheral hemoglobin, white blood cells and platelet counts in PDS-C groups with medium and higher doses were significantly higher than those in model control. Meanwhile, PDS-C ele-vated the percentages of Th2 cells and Treg cells, but decreased the percentage of Th1 cells, as well as up-regulated the GATA-3 , FOXP3 and down-regulated the T-bet protein levels. Conclusion PDS-C possesses the activities of promoting hematopoiesis obviously. It can improve marrow myelosuppression, enhance the re-covery of hematopoiestic function, and elevate the pe-ripheral blood cells counts. PDS-C also pays its immu-noregulatory efficacy though recovering from unbal-anced Th1/Th2/Treg cells in treating immune media-ted AA mice.
