Study of α1 adrenoceptor-mediated vasoconstriction and protein expressions induced by mmLDL in mouse mesenteric artery
10.3969/j.issn.1001-1978.2015.06.018
- VernacularTitle:mmLDL对小鼠肠系膜动脉α1受体介导的血管收缩及相关蛋白表达影响的研究
- Author:
Lijun GUO
;
Gaofeng JIANG
;
Haipeng LI
;
Qiong LI
;
Enqi LIU
;
Jie LI
- Publication Type:Journal Article
- Keywords:
mmLDL;
mesenteric artery;
α1 adreno-ceptor;
prazosin;
yohimbine;
tail intravenous injection
- From:
Chinese Pharmacological Bulletin
2015;(6):827-832,833
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of mmLDL on the up-regulation ofα1 receptors in moues mesenter-
ic arteries. Methods Mice tail intravenous injection of mmLDL was used . Vitro sensitive myograph was empl-
oyed to examine Noradrenaline ( NA) induced vascular contraction on mice mesenteric artery, and the mRNA and protein expressions ofα1 andα2 receptors were an-alyzed by real-time PCR and Western blot, respective-ly. Results mmLDL significantly increased NA in-duced concentration-contractile curve, and the data of Emax and pEC50 were from ( 122. 61 ± 9. 40 )% and (5. 65 ± 0. 05 ) in normal saline ( NS ) group to (161. 01 ± 6. 90 )% and ( 6. 20 ± 0. 08 ) in mmLDL group (P <0. 01, P <0. 01), respectively. The α1 adrenoceptor antagonist prazosin shifted the concentra-
tion-contractile curve induced by NA towards right. Af-ter using mmLDL, the mRNA and protein levels of α1 adrenoceptor were significantly increased, but the mR-NA and protein levels of α2 adrenoceptor were not changed. Conclusion Tail intravenous injection of mmLDL enhances the vascular expressions of α1 adre-noceptors and the contractile effects mediated byα1 ad-renoceptors.
