Study of reversal effect of PARP-1 inhibitor PJ34 on cisplatin-resistance in human lung adenocarcinoma cells
10.3969/j.issn.1001-1978.2015.06.025
- VernacularTitle:PJ34对肺腺癌顺铂耐药细胞增殖及耐药性的影响
- Author:
Yuanyuan YAO
;
Jiqing HAO
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
PARP-1 inhibi-tors;
cisplatin;
drug resistance;
apoptosis;
mechanism
- From:
Chinese Pharmacological Bulletin
2015;(6):865-869,870
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the reversal effect of PARP-1 inhibitor PJ34 on cisplatin-resistance in hu-man lung adenocarcinoma A549/DDP cells and the mechanism. Methods A549/DDP cells were treated with PJ34 alone or combined with cisplatin. The effects of proliferation inhibition were assayed by MTT meth-
od. The apoptosis ratios of cells were analyzed by flow cytometry. The protein expression of PARP-1 and LRP, GST-π were measured by Western blot assay. Results PJ34 could inhibit the proliferation of A549/DDP cells alone. The non-toxic dose of PJ34 could signifi-cantly resensitize A 5 4 9/ DDP cells to cisplatin , induce
apoptotic,lower the expression of PARP-1 and resist-ance-associated protein LRP and GST-π. Conclusion PJ34 could inhibit the proliferation of A549/DDP cells and resensitize A549/DDP cells,partially reverse cisplatin-resistance in A549/DDP cells, with a proba-
ble mechanism relating to increased apoptotic rate,and lowered expression of PARP-1 and resistance-associat-ed protein LRP and GST-π.
