Effects of baicalin on cognitive function in global cerebral ischemia/reperfusion rats and associated mechanism
10.3969/j.issn.1001-1978.2015.06.013
- VernacularTitle:黄芩苷对全脑缺血/再灌注大鼠认知功能的影响及其机制研究
- Author:
Rong LI
;
Zhenhua LI
;
Junqing YANG
;
Beibei CHEN
;
Oumei CHENG
- Publication Type:Journal Article
- Keywords:
baicalin;
global cerebral ischemia reper-fusion;
cognitive function;
BrdU;
hippocampus;
COX-2
- From:
Chinese Pharmacological Bulletin
2015;(6):801-804,805
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of baicalin on cognitive function in global cerebral ischemia reper-fusion rats, and the probable mechanism involved. Methods Sixty male Sprague-Dawley(SD) rats were randomly divided into Sham operation group ( S group) , global cerebral ischemia reperfusion group ( I/R group) , global cerebral ischemia reperfusion + ba-icalin treatment group ( I/RB group) , twenty in each. Model was induced via the bilateral occlusion of the
common carotid arteries plus hemorrhagic hypotension. 12 h after reperfusion, rats in I/RB group were given baicalin (100 mg·kg-1 ) saline solution by intragas-tric administration twice per day for 7 days. Rats in S group and I/R group were given the corresponding dose of saline infusion at the same time. Morris water maze test was employed to detect spatial learning and memo-ry. BrdU immunohistochemistry was used to detect the proliferation of neural precursor cells ( NPCs ) in the
brain. Expression of COX-2 in the brain tissue was measured by Western blot. Results Compared to I/R group, baicalin improved spatial learning and memory damage ( P <0. 05 ) , increased the number of BrdU-positive cells in the DG and SVZ ( P<0. 01 ) , and re-duced the expression of COX-2 in the brain ( P <0. 01 ) . Conclusions Baicalin could ameliorate cog-
nitive function in the rats with global cerebral ischemia reperfusion, which might be associated with its inhibi-tory effects on the expression of COX-2 , thereby in-creasing the proliferation of NPCs in the brain.
