Astragalus polysaccharides protects against free fatty acid-induced human vascular endothelial cell dysfunction via AMPK-eNOS pathway
10.3969/j.issn.1000-4718.2015.07.027
- VernacularTitle:黄芪多糖通过活化 AMPK-eNOS 信号通路减轻游离脂肪酸对人血管内皮细胞的损伤
- Author:
Jie SONG
;
Yangqian HU
;
Jian LIU
;
Jing LI
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharides;
AMP-activated protein kinases;
Endothelial nitric oxide synthase;
Free fatty acid;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
2015;(7):1305-1308,1314
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To study the protective effect of Astragalus polysaccharides ( APS) on free fatty acid-induced injury in human umbilical vein endothelial cells (HUVECs).METHODS: Cultured HUVECs were divided into control group, APS group [ APS (200 mg/L) treated for 24 h], free fatty acid group [free fatty acid (0.25 mmol/L) treated for 24 h], free fatty acid plus APS group [free fatty acid (0.25 mmol/L) and APS (200 mg/L) treated for 24 h], and com-pound C group [ free fatty acid (0.25 mmol/L) and APS (200 mg/L) and AMPK inhibitor compound C (10 μmol/L) treated for 24 h] .The cell viability was detected by MTT assay.Nitric oxide ( NO) content in the medium was determined by nitrate reductase assay.The protein levels of total adenosine monophosphate-activated protein kinase (AMPK), phos-phorylated adenosine monophosphate-activated protein kinase (p-AMPK), endothelial nitric oxide synthase (eNOS) and phosphorylated endothelial nitric oxide synthase ( p-eNOS) were measured by Western blot.RESULTS: No significant difference of all indexes between APS group and control group was observed.The cell viability in free fatty acid group de-creased significantly compared with control group.The cell viability in free fatty acid plus APS group was significantly im-proved as compared with free fatty acid group.The cell viability in compound C group was almost the same as that in free fatty acid group.The No content and protein levels of p-AMPK and p-eNOS in free fatty acid group decreased obviously as compared with control group, while the NO content and protein levels of p-AMPK and p-eNOS in free fatty acid plus APS group increased obviously compared with free fatty acid group.No significant difference of the p-AMPK and p-eNOS protein levels between free fatty acid plus APS group and free fatty acid group was observed.No significant difference of the AMPK and eNOS protein levels in all groups was found.CONCLUSION:APS attenuates the free fatty acid-induced injury, and its mechanism is related to the AMPK-eNOS signal pathway.
