Effect of fraxetin on cardiomyocyte hypertrophy induced by phenylephrine
10.3969/j.issn.1671-8348.2015.02.010
- VernacularTitle:秦皮素对苯肾上腺素诱导的大鼠心肌细胞肥大的影响
- Author:
Kuoju CHENG
;
Sheng JING
- Publication Type:Journal Article
- Keywords:
cardiomyocytes;
deoxye pine phrine;
fraxetin;
Nrf2
- From:
Chongqing Medicine
2015;(2):174-176,179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers (ANP ,BNP) mRNA expression levels of cardiomyyocyte hypertrophy .Results (1)Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;(2)The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .
