Exppresion of miRNAs target regulating FASN in different invasion ability osteosarcoma cells
10.3969/j.issn.1671-8348.2015.01.003
- VernacularTitle:靶向调控FASN的miRNAs在不同侵袭力骨肉瘤细胞中的表达
- Author:
Heng WANG
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
MicroRNAs;
transfection;
fatty acid synthase;
targeting regulation
- From:
Chongqing Medicine
2015;(1):7-9,13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the exppresion level of miRNAs which may target fatty acid synthase(FASN) in osteosarcoma (OS) cells of diverse invasion ability ,and to select the miRNAs target regulating FASN ,acting as the basis to investigate the mech‐anism of OS metastasis .Methods The miRNA and microrna .org online software were adopted to forecast the miRNAs that might target regulate FASN(NM_004104);RT‐PCR was used to detect the expression level of the predicted miRNAs in HOS and U2‐OS cells ;the protein expression of FASN was detected by Western blot ;Transwell invasion assay was used to evaluated the invasive a‐bility of HOS and U2‐OS cells .Results Two prediction methods all showed that miR‐195/15a/15b/16/424/497 molecular might be target FASN gene;RT‐PCR result showed that the expression level of miR‐424 in HOS cells was significantly higher than that in U2‐OS cells ;the expression level of FASN was significantly higher in U2‐OS cells than that in HOS cells ;the invasive ability of U2‐OS cells is significantly higher than HOS cells .Conclusion The expression level of miR‐424 in OS cells may be negatively related to the invasive ability ,and miR‐424 likely may affect OS metastasis via targeting FASN .
