Experimental study of cytokines in gastric mucosa of mice with Helicobacter pylori infection
10.3969/j.issn.1671-8348.2015.03.002
- VernacularTitle:幽门螺杆菌感染小鼠胃黏膜组织细胞因子变化的实验研究
- Author:
Hao LIU
;
Kaixue RUAN
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
gastric mucosa;
cytokines;
infection
- From:
Chongqing Medicine
2015;(3):293-294,298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of cytokines in gastric mucosa of mice with Helicobacter pylori infection and explore the immune mechanism .Methods Twenty‐four 8-10 weeks‐old BALB/c mice were randomly divided into observation group and control group .After first 12 hours of fasting ,observation group was given 0 .5 mL/d Helicobacter pylori bacterial liquid with con‐centration of 2 × 109 CFU/mL for 7 consecutive days .The control group was fed with 0 .5 mL/d saline for 7 consecutive days .Then the mice were infected with Helicobacter pylori gast model was established .Using Real‐time PCR to detect the expressions of IFN‐γ ,TNF‐α,IL‐8 and IL‐10 in murine plasma within 2 ,4 weeks after infection .And after 4 weeks of infection ,mice were sacrificed , and their stomach tissue sterile were separated .ELISA was used to detect the levels of IFN‐γ,TNF‐α,IL‐8 ,and IL‐10 mRNA in gastric mucosa .Results There was significantly difference between groups 2 weeks after infection(P<0 .05) .With lastingness of infection ,the expression and the mRNA of IFN‐γ,TNF‐α,IL‐8 were significantly increased ,and the rate of increase of observation group was higher(P<0 .05) .Conclusion TH2 cells may have no effect on Helicobacter pylori infection in mice gastric mucosa and promote the expression of T H1‐type cytokines ,triggering immune response of T H1 .
