Role of RIP140 and PGC-1αin angiotensin Ⅱ mediated energy metabolism in cardiomyocytes
10.3969/j.issn.1001-1978.2015.02.010
- VernacularTitle:RIP140/PGC-1α在AngII调节心肌能量代谢中的作用研究
- Author:
Yanfang CHEN
;
Peiqing LIU
- Publication Type:Journal Article
- Keywords:
angiotensin Ⅱ;
receptor interacting pro-tein 140;
peroxisome proliferator-activated receptor gamma coactivator-1α;
ATP;
energy metabolism;
car-diomyocytes
- From:
Chinese Pharmacological Bulletin
2015;(2):194-197,198
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of transcrip-tional cofactors receptor interacting protein 140 ( RIP140 ) and peroxisome proliferator-activated recep-tor γ coactivator-1α ( PGC-1α) in the angiotensin Ⅱ( AngⅡ) mediated energy metabolism regulation in cardiomyocytes. Methods RIP140 or PGC-1α was overexpressed via adenovirus vector system. ATP con-tents were detected by luminescence detection assay system. Real-time PCR and Western blot analysis were used to measure the expressions of RIP140 and PGC-1α genes. Results After treated with 100 nmol·L-1 AngⅡfor 36h in neonatal cardiomyocytes, the content of mitochondrial ATP was reduced notably ( P <0. 01). Accordingly, the mRNA and protein levels for RIP140 were increased. However, the mRNA and pro-tein levels of PGC-1α were downregulated markedly. What’ s more,the reduction of ATP induced by AngⅡwas further aggravated by RIP 1 4 0 overexpression , but ameliorated by overexpressing PGC-1α. Conclusion The reduction of ATP mediated by AngⅡis associated with the upregulation of RIP140 , as well as the down-regulation of PGC-1α.
