Research on mechanisms of PD-induced inhibition of adhesion, invasion and migration in non-small cell lung cancer H460 and A549 cells
10.3969/j.issn.1001-1978.2015.02.019
- VernacularTitle:桔梗皂苷-D抑制非小细胞肺癌H460和A549细胞黏附、侵袭和迁移的作用机制研究
- Author:
Ruolin ZHAO
;
Kunfu ZHOU
;
Xu ZHANG
;
Meijuan CHEN
- Publication Type:Journal Article
- Keywords:
platycodin-D;
non-small cell lung canc-er;
adhesion;
invasion;
migration;
matrix metallopro-teinase-2 ( MMP-2 );
matrix metalloproteinase-9 ( MMP-9)
- From:
Chinese Pharmacological Bulletin
2015;(2):241-246,247
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of the inhibition of cell adhesion , invasion and migration in non-small cell lung cancer ( NSCLC ) H460 and A549 cells in-duced by platycodin-D ( PD ) and its mechanism. Methods Cell adhesion assay, wound-healing assay and Transwell chamber migration assay were used to detect the ability of cell adhesion, migration and inva-sion. Regulation of MMP-2 and MMP-9 mRNA was de-termined by RT-PCR. Meanwhile, Western blot was performed to determine the expression levels of MMP-2/9 , its upstream related proteins of ERK signaling pathway and p-Akt. Results PD effectively inhibited the ability of cell adhesion, invasion and migration in H460 and A549 cells in a dose-dependent manner ( P<0. 05 ) . PD reduced the expression of MMP-2 and MMP-9 mRNA in H460 and A549 cells ( P<0. 01 );meanwhile, PD down-regulated the expression levels of MMP-2/9 , and inhibited the expression of its upstream proteins Ras, p-c-Raf, p-ERK 1/2 and p-Akt in a time- and dose-dependent manner. Conclusions PD inhibits cell adhesion, invasion and migration in NSCLC cells, and these effects are related to the down-regulation of the expression of MMP-2 and MMP-9 mR-NA and protein, and inhibition of its upstream expres-sion of ERK signaling pathway and p-Akt.
