The experimental study of anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in rabbit ears model
- VernacularTitle:抗炎因子TSG-6抑制兔耳瘢痕增生的实验研究
- Author:
Hui WANG
;
Xiaojing LI
;
Zhao CHEN
- Publication Type:Journal Article
- Keywords:
animal model;
hypertrophic scar;
rabbit;
inflammation;
TSG-6
- From:
Acta Universitatis Medicinalis Anhui
2015;(1):45-48,49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of tumor necrosis factorαstimulated gene-6 ( TSG-6 ) on hypertrophic scarring by using a rabbit ear model. Methods TSG-6 and PBS were injected intradermally in the right and left ear wounds, respectively. Collagen I and III expression detected by immunohistochemistry and scar elevation index ( SEI) was used to evaluate the extent of scarring. The expression of inflammatory factors interleukin-1β( IL-1β) , interleukin-6 ( IL-6 ) and tumor necrosis factor-α( TNF-α) was detected by immunohistochemistry and reverse tran-scription polymerase chain reaction. Transmission electron microscope ( TEM) and TUNEL analyses were used to detect fibroblast apoptosis. Results Compared with control scars, TSG-6-treated wounds exhibited decreased in-flammation significantly as evidenced by the lower levels of IL-1β, IL-6 , TNF-α. The apoptosis rate was higher and the SEI and the synthesis of collagens I and III were significantly decreased in the TSG-6-treated scars ( P<0. 05 ) . Conclusion Immediate topical injection of TSG-6 during the wound healing process can reduce the severity of hy-pertrophic scarring in a rabbit model. The anti-cicatrix effect of TSG-6 may result from controlling inflammation, in-ducing fibroblast apoptosis and promoting collagen degradation.
