Simultaneous Determination of the Contents of Dictamnolactone and Skimmianine in Root of Zanthoxylum armatum DC. from Gansu Province by HPLC
10.3969/j.issn.1005-5304.2015.02.025
- VernacularTitle:HPLC同时测定甘肃产竹叶椒根中白藓碱和茵芋碱含量
- Author:
Wenping LI
- Publication Type:Journal Article
- Keywords:
root of Zanthoxylum armatum DC.;
dictamnolactone;
skimmianine;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(2):91-92,93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for the simultaneous determination of the contents of dictamnolactone and skimmianine in the root of Zanthoxylum armatum DC. from Gansu Province. Methods The column was kromasil-C18 (250 mm×4.6 mm, 5 μm);the mobile phase was acetonitrile-water (65∶35);UV detection was performed at 254 nm;the flow rate was 1.0 mL/min;detected drift tube temperature was set at 30 ℃. Results The calibration curves were linear in the range of 0.205-2.05μg for dictamnolactone (r=0.999 9) and 0.199 6-1.996μg for skimmianine (r=0.999 7). The average recovery was 99.69%(RSD=0.57%) for dictamnolactone and 99.49% (RSD=1.04%) for skimmianine. Conclusion The method is simple and accurate. It is suitable for content determination of dictamnolactone and skimmianine in the root of Zanthoxylum armatum DC..
