Effect of resveratrol pretreatment on proliferation of cortical neural stem cells after oxygen-glucose deprivation/reoxygenation injury in rats
10.3969/j.issn.1001-1978.2015.01.024
- VernacularTitle:白藜芦醇预处理对氧糖剥夺/再复氧损伤大鼠皮质神经干细胞增殖的影响
- Author:
Wei CHENG
;
Changbo SHEN
;
Li WANG
;
Pingping YU
;
Qin YANG
- Publication Type:Journal Article
- Keywords:
resveratrol;
different concentrations;
pre-treatment;
neural stem cells;
oxygen-glucose depriva-tion;
reoxygenation injury;
proliferation
- From:
Chinese Pharmacological Bulletin
2015;(1):113-117,118
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the proliferative effect of resveratrol pretreatment on oxygen-glucose deprivation/reoxygenation ( OGD/R ) injury of rat cortical neural stem cells ( NSCs ) in vitro. Methods Isolation and purification of NSCs in neonatal Sprague-Dawley( SD) rats were conducted by suspended cultivation. The third passage NSCs of adherent culture was cultured under oxygen and glucose deprivation for 150 min and reoxygenation for 24 h. The experimental subjects were divided into normal, control, ethanol and resveratrol pretreatment groups. Immunofluorescence was used to identify NSCs. Cell viability was detected with CCK-8 assay. Flow cytometry cell cycle and BrdU assay were used to measure cell proliferation. Results Cells both in suspended and adherent cultivation highly expressed neuroepithelial stem cell protein ( nestin ) . Compared with the control group, NSCs viabilities and prolifera-tion in resveratrol groups (1, 5, 20 μmol·L-1 ) were significantly heightened, and highest in the 5 μmol · L-1 resveratrol group ( P<0. 05 ) . Conclusion Res-veratrol pretreatment can reduce injury and promote proliferation of NSCs after oxygen-glucose deprivation /reoxygenation.
