Determination of Three Sulfonamides Residues in Animal Derived Food by UPLC-MS/MS
- VernacularTitle:UPLC-MS/MS法测定动物源性食品中3种磺胺类药物残留
- Author:
Xingcui LI
;
Baoming LI
;
Yaping TIAN
- Publication Type:Journal Article
- Keywords:
UPLC-MS/ MS;
Sulfonamides;
Residue;
Animal derived food
- From:
China Pharmacist
2015;(1):38-40,41
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method for sulfadoxine, sulfathiazole and sulfadiazine in animal derived prod-ucts by ultra-performance liquid chromatography-tandem mass spectrometry ( UPLC-MS/MS) . Methods:Trichloroacetic acid and ace-tonitrile were used in the extraction and removal of proteins. A Waters Acquity UPLC BEH C18 (100 mm × 2. 1 mm,1. 7 μm) column was used as the separation column. The mobile phase was 0. 1% formic acid in water-methanol with gradient elution. The three sulfona-mides residues were detected with multiple reaction monitoring( MRM) mode in multi-period. Results:Under the conditions,the linear range of sulfadoxine, sulfathiazole and sulfadiazine was 1. 0-200. 0 ng·ml-1 ,the linear correlation coefficient was above 0. 99,the re-covery was more than 70%,the detection limit was 0. 5 ng·ml-1 , and the quantification limit was 1. 0 ng·ml-1 . Conclusion: The sample preparation method is simple and fast, and the method can be used to analyze sulfadoxine, sulfathiazole and sulfadiazine in ani-mal derived food efficiently and sensitively.
