Immunomodulatory effects of sea cucumber fucoidan on macrophage and the signaling pathways
10.3969/j.issn.1001-1978.2015.01.019
- VernacularTitle:海参岩藻聚糖硫酸酯对巨噬细胞的调节作用及信号通路研究
- Author:
Qi ZHANG
;
Xuemin LI
;
Zhaojie LI
;
Tao ZUO
;
Qingjuan TANG
;
Yaoguang CHANG
;
Jingfeng WANG
;
Changhu XUE
- Publication Type:Journal Article
- Keywords:
sea cucumber fucoidan ( SC-FUC);
mac-rophage;
NO;
IL-6;
IL-10;
Toll-like receptors
- From:
Chinese Pharmacological Bulletin
2015;(1):87-91,92
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the immunomodulatory effects of sea cucumber fucoidan ( SC-FUC) on macro-phage and the signaling pathways. Methods Cell via-bilities in response to different concentrations of SC-FUC were analyzed by MTT, phagocytosis ability was detected by neutral red,and nitric oxide ( NO) produc-tion was examined by Griess reaction kit. The mRNA expression levels of IL-6 , IL-10 , Toll-like receptors (TLRs) and related signal molecules MyD88, TRIF, NF-κB were assayed by real-time PCR. All the experi-ments were based on murine RAW264. 7 cell line. Re-sults SC-FUC could promote RAW264 . 7 cell prolif-eration, phagocytosis as evidenced by uptake of neutral red and release of NO. The effects were significant at the early stage (6 h and 12 h) . SC-FUC could up-reg-ulate the expression of IL-6 , IL-10 , TLR4 , TLR5 , TLR9. Moreover, mRNA expressions of TLRs signaling molecules were increased, as well as MyD88, TRIF, NF-κB. Conclusions SC-FUC could activate macro-phage, and then promote the immune function by pro-moting production or expression of NO, IL-6, IL-10. It is speculated to be relevant to activated cell surface re-ceptors in macrophage, including TLR4, TLR5, TLR9, and NF-κB signaling pathways.
