Hydroxysafflor yellow A inhibits VSMCs proliferation via PCNA and MEK-ERK1/2

VernacularTitle:羟基红花黄色素A通过影响PCNA表达和MEK-ERK1/2信号通路抑制大鼠血管平滑肌细胞增殖
Author: Jingshan ZHAO ; Mingxing FANG ; Qianyu GUO ; Yunfeng LI ; Bingyuan XU ; Shaohong LAI ; Shangyue ZHANG ; Yu LIU ; Aiying LI
Publication Type:Journal Article
Keywords: hydroxysafflor yellow A; VSMC; cell nu-clear antigen; extracellular signal regulated protein ki-nase; mitogen activated protein kinases; cell prolifera-tion
From: Chinese Pharmacological Bulletin 2015;(7):984-987,988
CountryChina
Language:Chinese
Abstract: Abstrac:Aim To study the effect of hydroxysafflor yellow A ( HYSA ) on the proliferation of vascular smooth muscle cells ( VSMCs) and the related molecu-lar mechanism. Methods The inhibitory effects of hydroxysafflor yellow A on VSMC proliferation was de-tected using cell culture, MTT assay, Western blot and immunohistochemical staining. Results The results showed that HYSA inhibited cell proliferation induced by PDGF in a dose-dependent (5,10,20,40 μmol· L-1 ) manner, reduced proliferating cell nuclear anti-gen ( PCNA ) expression and blocked PDGFR-MEK-ERK1/2 signaling pathway activated by PDGF in VSMCs. Conclusion HYSA inhibits VSMCs prolifer-ation via reducing the expression of PCNA and blocking signal transduction of MEK-ERK1/2 in VSMCs.