Application of antigen retrieval method in hMAM immunohistochemical staining of old paraffin-embedded specimens
10.7644/j.issn.1674-9960.2014.12.011
- VernacularTitle:抗原修复在陈旧石蜡切片乳腺珠蛋白免疫组化检测中的应用
- Author:
Cuimi DUAN
;
Xiqin YANG
;
Bingshui XIU
;
Zhiqiang LIU
;
Xuhui ZHANG
;
Zhenhua DAI
;
Haiping QUE
;
Xiaoyan FENG
;
Heqiu ZHANG
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
antigen retrieval;
human mammglobin;
immunohistochemistry;
paraffin;
microtomy
- From:
Military Medical Sciences
2014;(12):965-967,980
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an antigen retrieval method for detection of human mammaglobin ( hMAM) immuno-histochemcal staining in old paraffin-embedded specimens .Methods The tissue sections in test group were put into dis-tilled water after deparaffinization and then moved into citric acid buffer ( pH 3.5) for 10-15 min.The other two meth-ods,microwave method and high pressure cooker method ,were compared as control groups at the same time .Finally, immu-nohistochemistry SP method was used to check the antibody in the sections .Results The color appearance in the test group (pH 3.5 citric solution) was better than that of microwave oven and high pressure cooker groups .In the test group, tissue sections were not easily cast off from the slices .Conclusion In this study,we have established a new and simple antigen retrieval method which will contribute to immunohistochemistry technology .
