Primary research of human bone marrow mesenchymal stem cells transfected by Smad7

VernacularTitle:Smad7转染人骨髓间充质干细胞的初步实验研究
Author: Kang CHEN ; Xiaofeng CAI ; Yao TUO ; Jia LAI ; Xuqin LI
Publication Type:Journal Article
Keywords: receptors,transforming growth factor bata; trabeculectomy; Smad7; bone marrow mesenchymal stem cells
From: Chongqing Medicine 2015;(6):730-731,735
CountryChina
Language:Chinese
Abstract: Objective Human bone marrow mesenchymal stem cells were transfected by Smad7 and labeled with green fluores-cent mark.BMMSCs were implanted into rabbit glaucoma operational model and observed surviving condition.Methods Through BP and LR reaction,Smad7 with green fluorescent mark was inserted into human bone marrow mesenchymal stem cells by bacterio-phage,filtered positive colony and picked out cell line.15 New Zealand white rabbits were enforced trabeculectomy with BMMSC, then following up cell survival condition.Results Smad7 expressed stable in human bone marrow mesenchymal stem cells with sat-isfactory green fluorescent mark.BMMSC survived in rabbit trabecula with stable green fluorescent and effective ocular press.Con-clusion Smad7 with green fluorescent mark could be inserted into human bone marrow mesenchymal stem cells stably,and has ef-fective results in rabbit model.