Study on the biological characteristics of human breast cancer cell mammospheres

VernacularTitle:人乳腺癌细胞微球体生物学特性研究
Author: Yuanming FAN ; Jing HOU ; Ning DONG ; Qiang WANG ; Min GU
Publication Type:Journal Article
Keywords: breast neoplasms; neoplastic stem cell; culture in serum-free media
From: Chongqing Medicine 2015;(4):456-458,461
CountryChina
Language:Chinese
Abstract: Objective To study the biological characteristics of the human breast cancer cell mammospheres (MSs) ,and con‐struct breast cancer stem cell experiment model .Methods MCF‐7 cells were cultured in the serum‐free media supplemented with growth factors (the MCF‐7 group) ,and the MSs was collected (the MSs group) .The migration ,invasive and animal tumor forma‐tion abilities of MSs were detected by wound healing ,transwell invasive assay and animal tumor formation test .Results The wound line of MSs healed after 48 hours ,but the line of MCF‐7cells could not heal after 48 h .The number of the cells going through the membrane in MSs group was (76 .24 ± 0 .35) ,and the number in MCF‐7cells was (17 .38 ± 0 .18)(P<0 .05) .MSs had stronger ani‐mal tumor formation ability than MCF‐7 cells .Conclusion MSs have stronger abilities in migration ,invasive and animal tumor for‐mation ,and could be used in the studies of breast cancer stem cell as experimental model .