A Genome-wide Analysis of DNA Methylation in APP/PS1 Transgenic Mice by Methylation Microarrays
- VernacularTitle:甲基化芯片检测APP/PS1双转基因小鼠基因组DNA甲基化分布
- Author:
Lin CONG
;
Nannan ZHANG
;
Jianfei NAO
;
Yan REN
- Publication Type:Journal Article
- Keywords:
Alzheimer′s disease;
genome-wide;
DNA methylation;
APP/PS1 transgenic mice
- From:
Journal of China Medical University
2015;(2):160-163,188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution pattern of genome?wide CpG methylation in the animal model of APP/PS1 transgenic mice with Alzheimer′s disease(AD). Methods This study investigated the genome?wide DNA methylation profiles in the cortex tissues using methylat?ed DNA immunoprecipitation(MeDIP)combined with high?throughput sequencing. Results The analysis revealed 2 346 CpG sites existed only in AD mice,representing 485 unique genes as potentially associated with AD and these methylated DNA fragments distributed in different chromo?somes. Some hyper?methylated genes displayed familial aggregation. Conclusion There is significant difference in DNA methylation sites between APP/PS1 transgenic AD mice and corresponding wild mice,suggesting that DNA methylation may be involved in onset and development of AD.
