The effects of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells
10.3969/j.issn.1001-3733.2015.01.03
- VernacularTitle:p75NTR 对舌鳞癌 Tca8113细胞凋亡的影响
- Author:
Zhaotao ZHANG
;
Dongdong TONG
;
Qing LI
;
Fenghe ZHANG
- Publication Type:Journal Article
- Keywords:
Tongue OSCC cells;
p75NTR;
siRNA
- From:
Journal of Practical Stomatology
2015;(1):15-18,19
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells. Methods:p75NTR +Tca8113 cells were isolated from Tca 8113 cell line and transfected by p75NTR siRNA using lipofectamine. p75NTR mRNA and protein expression was examined by real time RT-PCR and western blot respectively.Cell proliferation was stud-ied by MTT assay,cell apoptosis was examined by flow cytometry.Results:Proliferation of p75NTR + cells was faster than that of p75NTR - cells.Transfection of p75NTR siRNA inhibited p75NTR mRNA and protein expression in p75NTR + Tca8113 cells,inhibi-ted the proliferation and increased the apoptosis of the cells.Conclusion:p75NTR gene plays a role in the apoptosis of Tca8113 cells.
