Polyclonal antibody specific for hypervariable region of VP1 capsid protein of Taura syndrome virus (TSV) prepartion and characteristic analysis
10.3969/j.issn.1000-484X.2015.02.017
- VernacularTitle:桃拉综合征病毒VP1高变区蛋白抗体制备及初步特性分析
- Author:
Xinxin HUANG
;
Chengang YUAN
;
Xue NING
;
Ming GU
;
Qiang CAI
;
Rui LIU
;
Chengping LU
- Publication Type:Journal Article
- Keywords:
Taura syndrome virus ( TSV);
VP1 protein;
Polycolonal antibody;
Blocking reaction
- From:
Chinese Journal of Immunology
2015;(2):221-224,229
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Preparation and immune characteristic analysis of polyclonal antibody against hypervariable region protein of Taura syndrome virus major capsid protein VP 1 as a reference for studies on immunological diagnosis reagent.Methods:The recombinant vector pET-VP1 was transformed into E.coli BL21 for protein expression.Immunizing a New Zealand rabbit with purified VP1 protein,the titer of anti-VP1 serum was determined by Agar diffusion test and ELISA.Monoclonal phage specific binding to the purified VP1 protein was used for competitive inhibition test.Results: The VP1 protein was soluble and high expression in E.coli BL21.The biological activity titer of anti-VP1 serum reached 1∶26 ,1∶217 determined by Agar diffusion test and ELISA respectively.A litter binding activity of antiserum and VP 1 protein could be blocked by monoclonal phage , but would not affect the final positive result.Conclusion:High titer antibody Preparation of the VP 1 hypervariable region protein.The binding activity of the polyclonal antibody with VP1 protein was not affected by the mutations of VP 1 protein in minority areas ,so the antiserum could be used as immu-nological detection diagnosis agent.
