Invasion and metastasis inhibition of Foxp3 on ovarian cancer cells Skov3 and its mechanism
10.3969/j.issn.1000-484X.2015.02.007
- VernacularTitle:上调Foxp3抑制卵巢癌细胞Skov3侵袭和迁移及其机制的研究
- Author:
Yongbi YANG
;
Lin LAI
- Publication Type:Journal Article
- Keywords:
Ovarian cancer;
Foxp3 gene;
Invasion;
Metastasis
- From:
Chinese Journal of Immunology
2015;(2):173-175,179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Foxp3 on the invasion and metastasis of the ovarian cancer cells Skov 3 and the related mechanism.Methods: Plasmid overexpressing Foxp 3 gene was constructed and transfected into Skov 3 cells using liposome.Proliferation,invasion,RT-PCR and Western blot analysis were done to observe the expression of Foxp 3 gene in Skov3 cells and the invasion and metastasis ability.Also,MMP-2 and MMP-9 gene expression was detected.Results:Overexpression of Foxp3 was confirmed in Skov3 cells after Foxp3 plasmid transfection.Compared with the empty vector group , overexpression of Foxp3 gene significantly inhibited the migration and invasion of Skov 3 cells.Meanwhile , the expression level of MMP-2 and MMP-9 genes were decreased.Conclusion:Upregulation of FoxP3 gene expression can inhibit cell proliferation ,invasion and migration in ovarian cancer Skov3 cells through downregulating MMP-2 and MMP-9 gene expression.This study provided experimental basis for further research on Foxp3 gene and gene therapy for ovarian cancer.
