Expression and significance of Gab2 in colorectal cancer tissues
10.3969/j.issn.1000-484X.2015.02.004
- VernacularTitle:Gab2在结直肠癌组织中的表达及其意义
- Author:
Chenbo DING
;
Jihong FENG
;
Weina YU
;
Shaoying GAO
;
Lin XU
;
Chao CHEN
;
Junmin LUO
- Publication Type:Journal Article
- Keywords:
Gab2;
Colorectal cancer;
Immunohistochemistry;
Western blot
- From:
Chinese Journal of Immunology
2015;(2):160-162,168
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and significance of Gab 2 in colorectal cancer tissues .Methods:Immuno-histochemistry was used to detect the expression of Gab 2 in 78 cases of colorectal cancer tissues and 46 cases of the adjacent tissues and to analyze the association of Gab2 expression with the clinicopathologic features of colorectal cancer;The expression of Gab2 in samples from 10 cases of colorectal cancer tissues and matched adjacent nontumorous tissues was detected by Western blot .Results: The results of immunohistochemistry demonstrated that the Gab 2 protein positive expression rate in 78 cases of colorectal cancer was 53.85%;whereas was negative expression or weak in the adjacent tissues , showing a significant difference of comparison within this result (P<0.001) .The expression of Gab2 in colorectal cancer was related with TNM stage and lymph node metastasis (P<0.05) , but no relation with the size and differentiation of tumor (P>0.05) .Western blot showed that the Gab2 protein expression of colorectal cancer cases was significantly higher than that of matched adjacent nontumorous tissues ( P<0.05 ).Conclusion: Gab2 was overexpressed in colorectal cancer .Gab2 maybe play an important role in carcinogenesis and progression of colorectal carcinoma .
