Inducing effect of 4-Amino-2-Trifluoromethyl-Phenyl Retinate on differentiation of human breast cancer MDA-MB-231 cell and its possible mechanisms
10.3969/j.issn.1001-1978.2015.07.017
- VernacularTitle:4-氨基-2-三氟甲基苯基维甲酸酯对人乳腺癌细胞株MDA-MB-231诱导分化作用及可能的机制研究
- Author:
Jing LEI
;
Feihu CHEN
;
Jinfang GE
;
Yue LI
;
Wenfan GAO
;
Ziyun DENG
- Publication Type:Journal Article
- Keywords:
ATRA;
ATPR;
differentiation;
MDA-MB-231 cells;
RARs;
RXRs;
ERs
- From:
Chinese Pharmacological Bulletin
2015;(7):973-978,979
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of 4-Amino- 2-Trifluoromethyl-Phenyl Retinate on human breast cancer cells MDA-MB-231 and the possible mecha-nisms. Method Human breast cancer MDA-MB-231 cells were incubated with different concentrations of ATPR in vitro. MTT assay was performed to measure the proliferation of MDA-MB-231 . Cell growth curves were made by counting cells and morphologic changes were observed by Wright-Giemsa staining. The differ-entiation marker mucin-1 ( MUC-1 ) was measured by enzyme linked immunosorbent assay ( ELISA ) . Cell cycle was examined by Flow cytometry ( FCM ) . The expression of retinoic acid receptors ( RARs) and reti-noid X receptors ( RXRs ) were detected by Western blot and Quantitative real-time PCR (q-RT-PCR),re-spectively. Results Compared with solvent group, ATPR could inhibit the proliferation of MDA-MB-231 cells in a time-and dose dependent manner and induce the maturing and normality of morphology. The express of MUC-1 was significantly decreased, and the progres of cell cycle was blocked in the G0/G1-phase. The ex-pression of RARγ was decreased. Conclusions AT-PR could inhibit proliferation and induce differention of MDA-MB-231cells, it′s associated with RARγ.
