Neuroprotective effects of YXETNZ injection on SH-SY5 Y cells against injury induced by oxygen-glucose deprivation
10.3969/j.issn.1001-1978.2015.07.021
- VernacularTitle:银杏二萜内酯葡胺注射液对缺糖缺氧损伤的SH-SY5 Y细胞保护作用
- Author:
Qiu LIU
;
Zhiliang XU
;
Jun ZHOU
;
Na LI
;
Yuan BI
;
Zhenzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
YXETNZ injection;
oxygen-glucose dep-rivation;
apoptosis;
PKA;
Akt;
Bad
- From:
Chinese Pharmacological Bulletin
2015;(7):994-998,999
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects of YXETNZ injection on SH-SY5 Y cells damaged by oxygen-glucose deprivation ( OGD ) , and explore its functional mechanisms. Methods After 4 h of OGD, the cells were treated with 25 mg·L-1 drugs for 1 h. Subsequently, cell viabilities were measured by cell counting kit-8 ( CCK-8 kit ) and cell apoptosis was measured by caspase-3/7 assay kit according to manu-facturer’ s instructions. Furthermore, cell death was also detected by ELISA. The levels of phospho-Akt, phospho-PKA,phospho-Bad were evaluated by western blot. Results Oxygen-glucose deprivation significant-ly decreased the cell viabilities of SH-SY5Y cells, while YXETNZ injection significantly increased cell vi-abilities, phospho-Akt, phospho-PKA and phospho-Bad. Furthermore, YXETNZ injection also reduced the activities of caspase-3/7 and cytoplasmic histone-asso-ciated-DNA-fragments contents. Conclusion Our re-searches demonstrat that YXETNZ injection shows good neuroprotective effects on SH-SY5 Y cells after oxygen-glucose deprivation. The underlying mechanisms may be associated with activation of PI3 K/Akt/Bad/caspase-3/7 , cAMP/PKA/Bad/caspase-3/7 signaling pathway.
