Application of fluorescence PCR melting curve method in drug-resistance detection of Mycobacterium tuberculosis
10.3969/j.issn.1673-4130.2015.05.014
- VernacularTitle:荧光 PCR 熔解曲线法在结核分枝杆菌耐药检测中的临床应用
- Author:
Bingbing WANG
;
Mingri GUO
;
Hongxia XIAO
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
mycobacterium tuberculosis;
drug-resistant tuberculosis;
melting curve method
- From:
International Journal of Laboratory Medicine
2015;(5):608-609,612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical application value of fluorescence PCR melting curve method in the drug-resistance detection of Mycobacterium tuberculosis.Methods With the Lowenstein-Jensen culture method and the absolute concentration method as the comparison,the fluorescent PCR melting curve method was used to detect the resistance to rifampin and isoniazid in 53 cases of pulmonary tuberculosis(TB)respectively.Results The single drug resistance rates and multidrug resistance (MDR) rate detected by the two methods had no significant statistical difference(P >0.05).The comparison between the two methods for detecting the same sample showed that compared with the absolute concentration method,the sensitivity and specificity of the melt-ing curve method for detecting rifampin-resistance,isoniazid-resistance were 80.0% and 81.0%,100.0% and 93.8% respectively, the accuracies were 92.2% and 86.8% respectively,the Kappa values in the consistency analysis of the two methods were 0.754 and 0.710 respectively,indicating the good consistency between them.Conclusion Compared with the traditional methods,the melting curve method has good sensitivity and good specificity with safety,high efficiency and good application value in the treat-ment of drug-resistant pulmonary TB.
