Establishment and application of simple orthotopic kidney and bladder tumor models

VernacularTitle:简易肾癌及膀胱癌原位肿瘤模型的建立与应用研究
Author: Qi ZHANG ; Shanshan WANG ; Dexuan YANG ; Linna LI ; Jianghua LIU ; Pan KEVIN ; Shoujun YUAN
Publication Type:Journal Article
Keywords: kidney neoplasms; urinary neoplasms bladder; histopathology; models,animal; antineoplastic agents
From: Military Medical Sciences 2015;(7):537-540,545
CountryChina
Language:Chinese
Abstract: Objective To establish a simple and useful kidney or bladder orthotopic tumor model used in preclinical pharmacodynamic evaluation.Methods Mouse model of orthotopic renal cancer were established by subrenal capsule implantation.After aspirating urine and irrigating bladder with PBS,the bladder urothelium was slightly impaired to establish the orthotopic bladder tumor model.Then, B-Ultrasound and H&E staining were used to confirm the availability.Results Tumors could be seen 2 weeks after surgery, accompanied by body mass loss of the mice.H&E staining showed that the tumor cells acted as infiltrative growth.The growth of tumor was inhibited by NTX in vivo, the tumor mass inhibitory rate of the KCC-853 orthotopic tumor model was 57.5% of 60 mg/kg NTX treatment and 48.8% in the T24 orthotopic tumor model of 30 mg/kg NTX treatment.Conclusion Our methods for establishing the orthotopic kidney or bladder tumor model are simple and practical.The results indicate that nitroxoline has potential antitumor activity.