Effects of SAHA combined with PTX on lethality and autophagy of human ovarian cancer OC3 cells
10.7644/j.issn.1674-9960.2015.07.004
- VernacularTitle:辛二酰苯胺异羟肟酸联合紫杉醇对卵巢癌细胞OC3杀伤与自噬的影响
- Author:
Yue ZHAO
;
Yuanlin LIU
;
Zhaohui LIU
;
Si ZHANG
;
Ying TONG
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
SAHA;
PTX;
lethality;
autophagy;
human ovarian cancer cell OC3
- From:
Military Medical Sciences
2015;(7):504-507,513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of suberoylanilide hydroxamic acid(SAHA) or/and paclitaxel(PTX) on lethality and autophagy of human ovarian cancer OC3 cells,and to explore whether the combination of the two drugs has a synergistic function.Methods The morphology of OC3 cells was treated with SAHA and/or PTX, and then the morphology of treated OC3 cells was observed under an inverted microscope, cell proliferation was detected by MTT assay and autoph-agy was analyzed by AO/EB double staining assay.The synergistic effect of SAHA and/or PTX was analyzed by factorial design and gold formula method.Results After treatment with SAHA and/or PTX, the morphology of OC3 cells in the combination group ( SAHA+PTX) displayed significant morphological changes.OC3 cells became less adherent and refrac-tive than in other groups.Cell proliferation by MTT assay demonstrated that the growth inhibition rate of the combination groups was higher than in groups treated with SAHA or PTX respectively( P<0.05) .Furthermore, the synergistic effect af-ter treatment with a combination of SAHA with PTX was proved by the factorial design and gold formula method.The auto-phagy rate of the combined groups was significantly higher than in single treatment groups (P<0.05) by AO/EB double staining.Conclusion SAHA and PTX can inhibit the survival of OC3 cells and induce its autophagy.The two drugs have synergistic antitumor effects.
