Expression and significance of chemokine receptor CX3CR1 in rats with severe acute pancreatitis
10.7652/jdyxb201502008
- VernacularTitle:趋化因子受体 CX3CR1在大鼠重症急性胰腺炎中的表达和意义
- Author:
Chunping FU
;
Liya HUANG
;
Li YANG
;
Chunxiang SHI
;
Hui LI
;
Ping SHAO
- Publication Type:Journal Article
- Keywords:
chemokine;
CX3CR1;
severe acute pancreatitis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):186-189,279
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To explore the expression of chemokine receptor CX3CR1 in severe acute pancreatitis (SAP)rats model and to estimate whether it can be a potential predictor of SAP.Methods Forty Sprague-Dawley rats were randomly divided into sham-operation (SO)group (n =20)and SAP group (n =20).Rats were killed at 6 h,12 h,24 h and 48 h after model induction.The serum level of CX3CR1 was measured by enzyme-linked immunosorbent assay (ELISA).The expression of CX3CR1 protein in the pancreas,lung and kidney were detected by immunohistochemistry.Results The serum level of CX3CR1 in SAP rats increased gradually after model induction and reached the peak at 24 h (542.4 pg/mL),which were significantly higher than those in SO group (P <0.05).The expression of CX3CR1 was found in the pancreas,lung and kidney tissues of SAP rats and higher than that in SO group.Meanwhile,the expression of CX3CR1 reached the peak at 24 h in the pancreas and lung and at 48 h in the kidney.Conclusion This study suggests that the chemokine receptor CX3CR1 may be one effective index for predicting the severity of acute pancreatitis and deserves further research.
