Construction of recombinant adenovirus harboring fusion gene NT4p53(C22)Ant and its killing effect on HepG2 tumor cells
10.7652/jdyxb201502003
- VernacularTitle:NT4p53(C22)Ant融合基因重组腺病毒的构建及对肝癌细胞的杀伤作用
- Author:
Qi ZHOU
;
Xu SHANG
;
Yongbo XU
;
Jue JIANG
;
Hua WANG
;
Miao LI
- Publication Type:Journal Article
- Keywords:
NT4p53(C22)Ant;
recombinant adenovirus;
carcinoma;
gene therapy
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):159-163,168
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To construct a recombinant adenovirus vector harboring fusion gene NT4p53(C22)Ant and study its killing effect on HepG2 tumor cells.Methods Using molecular cloning technology,the rAVV-NT4p53(C22)Ant was produced by homologous recombination.Then we collected virus supernatant and measured its titer after it was amplified by PCR.The effect of this fusion gene on HepG2 tumor cells was evaluated by IHC, MTT assay,PI staining and flow cytometry.Results The recombinant adenovirus was successfully constructed. The p53 expression rate in rAAV-NT4p53(C22)Ant group was (44.88±2.45)%.MTT assay showed that rAAV-NT4p53(C22)Ant could strongly suppress the growth of HepG2 tumor cells.Flow cytometry showed that rAAV-NT4p53(C22)Ant could induce obvious apoptosis of HepG2 tumor cells.Conclusion The recombinant adenovirus vector encoding gene NT4p53(C22)Ant has been successfully constructed and expressed in this experiment,and it can inhibit proliferation and induce apoptosis of HepG2 tumor cells.
