The effect and mechanism of allicin on the migration and invasion properties of human osteosarcoma U87 cells
10.7652/jdyxb201502024
- VernacularTitle:大蒜素对脑胶质瘤细胞 U87细胞侵袭能力的影响及其机制
- Author:
Qing CAI
;
Huaizhou QIN
;
Kunlun CHEN
- Publication Type:Journal Article
- Keywords:
glioma;
allicin;
invasion;
matrix metalloproteinases (MMPs);
p38;
U87 cells
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):271-274,284
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms.Methods In this study,we employed MTT assay to test the anti-proliferative effect of allicin. Transwell assay was used to test the anti-metastatic ability of allicin.Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2 (MMP-2 ) and matrix metalloproteinase-9 (MMP-9).Western blotting was employed to test the phosphorylated level of p38.Results Allicin could significantly inhibit the proliferation and invasion of U87 cells (concentration>8 μg/mL,P <0.05). Meanwhile allicin (concentration<8μg/mL)could inhibit the invasion of U87 cells.After treatment with allicin for 24 hours,the expressions of MMP-2 and MMP-9 were decreased significantly (P < 0.05 ).Moreover,allicin treatment decreased the phosphorylated level of p38 obviously (P < 0.05 ).Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway,suggesting that allicin is a potential therapeutic agent for glioma.
