Expressions and significance of EGFR,Ku70,NF-κB and Bcl-2 in Graves’disease
10.7652/jdyxb201502014
- VernacularTitle:EGFR、Ku70、NF-κB 及 Bcl-2在 Graves 病中的表达及意义
- Author:
Kun GUO
;
Rui GAO
;
Yan YU
;
Weixiao ZHANG
;
Yan LIU
;
Aimin YANG
- Publication Type:Journal Article
- Keywords:
Graves’disease;
Ku70;
epidermal growth factor receptor (EGFR);
nuclear factor-kappa B (NF-κB);
Bcl-2
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):215-218,230
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To investigate the roles of Ku70,epidermal growth factor receptor (EGFR),nuclear factor-kappa B (NF-κB)and Bcl-2 genes in the pathogenesis of Graves’disease.Methods Fine needle biopsies of 59 patients [M:F 1 6:43;mean age (44.66 ± 12.94)y)]diagnosed as having Graves’disease and 27 [M:F 6:21;mean age (44.64± 14.27)y]control normal tissues were collected.The mRNA expressions of EGFR,Ku70,NF-κB,and Bcl-2 were detected by Real-time polymerase chain reaction (RT-PCR).The Student’s t test was performed to analyze differences between patients with Graves’disease and control subjects.Pearson correlation analysis was applied to detect the relationship between gene mRNA expression and serological indexes.Results Compared with those of control subjects,the mRNA expression levels of EGFR (0.859 ±0.125 vs .1.752 ±0.660,P <0.05)and Ku70 (0.768±0.102 vs .3.304±0.402,P <0.001)were significantly higher in Graves’disease patients.However, the mRNA expressions of Bcl-2 (1.235±0.261 vs .0.834±0.086,P <0.05)and NF-κB (0.578±0.066 vs .0.884 ±0.085,P <0.05)were much lower in Graves’disease patients.Furthermore,the mRNA expression of Bcl-2 was positively correlated with that of NF-κB (r =0.399,P <0.05 ).The Pearson correlation analysis showed that the higher expression of Ku70 was positively associated with the serum TgAb level (r = 0.263,P < 0.05 ),but expressions of the other target genes were not significantly related to the serological indexes (all P > 0.05 ). Conclusion The signal transduction pathways mediated by Ku70,EGFR,NF-κB and Bcl-2 may participate in the occurrence and development of Graves’disease.
