Application of flow cytometric score in the diagnosis of myelodysplastic syndromes

VernacularTitle:应用流式细胞术评分诊断骨髓增生异常综合征
Author: Hongyan WANG ; Mei CHENG ; Xiuli YANG ; Lijun CHEN ; Jun MA
Publication Type:Journal Article
Keywords: Myelodysplastic syndromes; Flow cytometry; Sensitivity; Specificity
From: Journal of Leukemia & Lymphoma 2014;23(9):530-533,537
CountryChina
Language:Chinese
Abstract: Objective To identify myelodysplastic syndromes (MDS) from non-MDS (mainly non-clonal cytopenias) by flow cytometric (FCM) score.Methods Four parameters (CD34+ myeloblast-related and B-progenitor-related cluster size,myeloblast CD45 expression and granulocyte side scatter value) were rated on 50 patients with MDS and 20 patients with non-clonal cytopenias.A diagnosis of MDS was formulated in the presence of FCM score value ≥ 2 (every abnormal parameter was scored 1).Results When compared with non-clonal cytopenias,patients with MDS had increased myeloblast-related cluster size and decreased B-progenitor-related cluster size,and had a lower granulocyte side scatter (P ＜ 0.05),but no significant difference was found when myeloblast CD45 expression was compared (P =0.06).The FCM score value was significant higher in MDS patients (P ＜ 0.001).45 cases were correctly classified (sensitivity 90 ％).There was 1 false-positive case among 20 controls (specificity 95 ％).Conclusion The FCM score may help to establish the diagnosis of MDS,and it is simple and feasible.