MiRNA-106a Induces Multidrug Resistance of Gastric Cancer Cells by Targeting RUNX 3

VernacularTitle:MiRNA-106a通过作用RUNX3基因诱导胃癌细胞多药耐受
Author: Yi ZHANG ; Xun CAI ; Weidong JIN
Publication Type:Journal Article
Keywords: gastric cancer; multidrug resistance; miRNA-106a; runt-related transcription factor 3
From: Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2015;(1):42-46,73
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of miRNA‐106a(miR‐106a) expression on multidrug resistance(MDR)of gastric cancer(GC)cells and the involvement of runt‐related transcription factor 3 RUNX3.Methods The expression of miR‐106a was detected in two human gastric adenocarcinoma cell lines with MDR by immunoblotting and apoptosis assay. The sensitivity of GC cells to anticancer drugs was observed by detecting the expression of miR‐106a by using immunoblotting and PCR ,and the relationship between miR‐106a and RUNX3 was determined by luciferase activity assay.Results miR‐106a was significantly in‐creased in GC cells with MDR ,and it suppressed the sensitivity of GC cells to anticancer drugs. It could modulate MDR by tar‐geting RUNX3.Conclusion miR‐106a can induce the MDR by targeting RUNX3 in GC.