The effect of S-Nitrosoglutathione on the intestinal epithelial barrier after acute ischemia reperfusion injury
10.3969/j.issn.1671-8348.2015.06.002
- VernacularTitle:S-亚硝基谷胱甘肽对小鼠肠急性缺血再灌注损伤后肠屏障功能的影响
- Author:
Ke PENG
;
Chao XU
;
Min YU
;
Lihua SUN
;
Weidong XIAO
;
Hua YANG
- Publication Type:Journal Article
- Keywords:
S-Nitrosoglutathione;
intestinal epithelial barrier;
tight junction protein;
intestinal ischemia reperfusion
- From:
Chongqing Medicine
2015;(6):724-726,729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of S-Nitrosoglutathione (GSNO)on acute ischemia reperfusion (I/R)induced in-testinal barrier function lesion in a mouse model.Methods Twenty-four 6-8-year-old C57BL/6 mice were divided into 3 groups,8 for each:(1)the sham group;(2)the I/R group;(3)the I/R+GSNO group.The mouse intestine I/R model was established by the occlusion of the superior mesenteric artery temporarily followed by reperfusion for 6 h.Histological changes in the small intestine were observed after HE staining;the expression of claudin-1 protein in the intestine epithelium was assessed by immunohistochem-istry staining as well as western blot analysis.Results Both HE staining and immunohistochemistry results showed the integrate intestinal villi with the continuous Claudin-1 expression alone the villi in the sham group;the intestinal villi of the I/R group partial-ly detached,thickened,crooked and fractured,with the obvious disconnection of Claudin-1 staining alone the top of the villi;while the intestinal villi of the I/R+GSNO group were neatly arranged and damage to intestinal mucosa was much alleviated,accompanied with the marked restoration of the continuity of claudin-1 staining.Compared to the sham group,claudin-1 protein level of for the I/R group and the I/R+GSNO group decreased by 32.5% and 13.8% respectively (P <0.05);and compared to the I/R group,clau-din-1 protein level of the I/R+GSNO group increased by 27.8% (P <0.05).Conclusion Protein level of claudin-1 would decrea-ses after I/R,and pretreatment with GSNO can effectively relieve the damage of intestinal mucosal structure as well as intestinal tight junction barrier through upregulating the expression of claudin-1 protein.
