Effects of SAA protein on biological behavior of nasopharyngeal carcinoma CNE2 cells
10.3969/j.issn.1671-8348.2015.06.006
- VernacularTitle:SAA 对鼻咽癌 CNE2细胞生物学行为的影响
- Author:
Qingshan JIANG
;
Shan DENG
;
Baoming SHEN
- Publication Type:Journal Article
- Keywords:
serum amyloid A protein;
nasopharyngeal neoplasms;
CNE2 cell;
transfection;
cell cycle
- From:
Chongqing Medicine
2015;(6):736-737,740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of over expression and inhibition expression of SAA protein on biological behavior of nasopharyngeal carcinoma CNE2 cells.Methods pcDNA3.1 (+)-SAA-CNE2 cell lines of high expression and pGPU6/GFP/Neo-SAA-CNE2 cell lines of interference expression of SAA protein in vitro.These two cells constructed by transfection of pcD-NA3.1(+)-SAA plasmid of SAA high expression and pGPU6/GFP/Neo-SAA plasmids of SAA inhibition expression respectively, plasmids of which were previously successfully reconstructed by the research group.Cell cycle of these two cells was analyzed by flow cytometry with PI staining.The ability of cell proliferation was inspected by plate cloning-forming test.Results Flow cytome-try showed that with the increase of expression of SAA protein,it had effect on promoting CNE2 cell division.Plate cloning-forming test showed that SAA protein can improve proliferation of the CNE2 cells.Conclusion SAA protein has the effect on promoting proliferation of human nasopharyngeal carcinoma CEN2 cell and migration in vitro.
