Fasudil relives cerebral vasospasm following subarachnoid hemorrhage by inhibiting TLR 4 expression
10.3969/j.issn.1671-8348.2015.05.003
- VernacularTitle:法舒地尔降低TLR4表达缓解蛛网膜下腔出血后血管痉挛的实验研究
- Author:
Li ZHANG
;
Fei LI
;
Weiwei PAN
;
Xiaoyun SUN
- Publication Type:Journal Article
- Keywords:
subarachnoid hemorrhage;
Toll-like receptor 4;
interleukin 10;
cerebral vasospasm;
Fasudil
- From:
Chongqing Medicine
2015;(5):583-585,589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Toll‐like receptor 4 inhibitor Fasudil on cerebral vasospasm after SAH .Methods A total of 60 New Zealand rabbits were randomly divided into three groups ,20 rabbits for each group ,SAH group:SAH model was established by autologous blood injection into the cisterna magna .Control group:saline was injected into cisterna magna .Fasudil group:Fasudil was injected into vein after SAH model .Vasospasm was valued by DSA and TCD .Seven days after operation basilar artery were collected .HE stain was used to observe vasospasm .TLR4 were observed by immunohistochemistry and western blot‐ting .Results Vasospasm model after SAH was successfully established .The basilar artery diameters were significantly shorter in the model group compared with the normal group (P<0 .01) .The artery diameter in Fasudil group increased significantly compared with SAH group(P<0 .01) .The expression of TLR4 decreased significantly in the Fasudil group compared with the model group (P<0 .05) .Conclusion Toll‐like receptor 4 pathway may be associated with cerebral vasospasm (DCV) .Fasudil could inhibit TLR‐4 expression and prevent cerebral vasospasm following subarachnoid hemorrhage .
