Experimental study on determination of viability of Schistosoma japonicum cercariae by staining
10.16250/j.32.1374.2014226
- VernacularTitle:染色法判别日本血吸虫尾蚴生命状态的实验研究
- Author:
Qinlu BAO
;
Chunlan HUANG
;
Dandan CHEN
;
Xuelong WANG
;
Shaosheng WANG
;
Xiaoniu TANG
;
Shulin ZHOU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Cercariae;
Staining method;
Viability;
Determination
- From:
Chinese Journal of Schistosomiasis Control
2015;(1):64-65,69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Schistosoma japonicum cercariae were stained by 0.4%trypan blue 0.5%methylene blue?eosin?borax M.E.B 0.5%eosin 0.5%methy?lene blue and 0.05% neutral red respectively for 5 min then they were observed under a stereoscopic microscope. Results The dead cercariae were stained in the trypan blue M.E.B eosin and neutral red but unstained in the methylene blue. The vi?tal cercariae were unstained in all the five kinds of dyes. Conclusion The staining methods by using 0.4% trypan blue 0.5%M.E.B 0.5%eosin and 0.05%neutral red can be used to determine the viability of S. japonicum cercariae.
