Targeting study of VEGFR-3 antibody loaded microbubble in vitro
- VernacularTitle:载VEGFR－3抗体淋巴管靶向微泡的体外寻靶试验研究
- Author:
Yang YANG
;
Mei ZHU
;
Zheng LIU
;
Xiaofeng ZHANG
;
Fei YAN
;
Xiyan ZHAO
- Publication Type:Journal Article
- Keywords:
VEGFR-3 antibody;
microbubbles;
target;
in vitro
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(3):10-12,17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare targeted microbubbles which load anti-VEGFR-3 and observe the targeting effect in vitro.Methods Targeted microbubbles loaded VEGFR-3 antibody were prepared and the targeting effect in vitro were tested by direct and indirect methods.Direct method took off-line analysis by image analysis software IPP and statistical analysed with SPSS software package.Indirect method took direct observation the target binding effect after incubation and centrifugal sedimentation.Results Image software IPP results showed that the quantity of microbubbles in targeted-group was obviously more than ordinary group.The average microbubbles per field in targeted-group was (151 ±20).The average microbubbles per field in ordinary group was ( 12 ±4 ) microbubbles, the numbers of combined microbubbles between targeted-group and ordinary-group had significant differences ( t=19.19,P<0.01).The indirect method results showed that, compared with ordinary group, the microbubbles in targeted-group could combined with cell gradually.Conclusion Targeted microbubbles load anti-VEGFR-3 have a better contrast effect than common microbubbles.
