Expression of α-SMA and PCNA in different vascular of OSAS with hypertension rat model
10.3969/j.issn.1006-5725.2015.05.005
- VernacularTitle:α-SMA和PCNA在OSAS继发高血压模型大鼠血管中的表达
- Author:
Jing CHEN
;
Yan ZHOU
;
Pengcheng XIA
;
Yiping GAO
- Publication Type:Journal Article
- Keywords:
Obstructive Sleep Apnea Syndrome;
Abdominal Aorta;
Renal Artery;
α-SMA;
PCNA
- From:
The Journal of Practical Medicine
2015;(5):708-710,711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and analyze the expression of α-SMA and PCNA in abdominal aorta and renal artery in obstructive sleep apnea syndrome(OSAS)combined with hypertension rats. Methods 36 SD rats were randomly divided into normal control group and model group. The model group was subjected to intermittent hypoxia condition for 8h everyday. Rat tail artery pressure was monitored every week and all subjects were sacrificed at 12th week. The level of TNF-α, IL-6, IL-10 and hs-CRP expression in serum were measured by ELISA method. Immunohistochemical analysis was performed on α-smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA). Results Compared with control group,model rats blood pressure increased significantly (P < 0.01) when exposed for 4 weeks under intermittent hypoxia condition,and reached the peak value of 186mmHg at 12 weeks. The level of TNF-α, IL-6 , IL-10 and hs-CRP in serum were higher than in control group (P < 0.01). Immunohistochemical staining showed that the expression of α-SMA in abdominal aorta decreased more significantly than control group (P < 0.05),while there was a stronger positive expression of PCNA in model group than those in control (P < 0.05). In addition,compared with control rats, model rats showed a higher expression of α-SMA and PCNA significantly in renal artery(P < 0.05). Conclusion The intermittent hypoxia condition could result in higher blood pressure,while the different expression of α-SMA and PCNA illustrate that high pressure show different reconstruction performance in different vascular,underlying different molecular mechanism.
