Determining the ideal decellularization protocol applied for preparing for liver biological scaffold
10.3969/j.issn.1671-8348.2015.09.001
- VernacularTitle:选择适宜的灌注去细胞方法应用于全肝脏去细胞支架的制备
- Author:
Kanghua WANG
;
He HONG
- Publication Type:Journal Article
- Keywords:
decellularization;
tissue engineering;
scaffold;
protocol
- From:
Chongqing Medicine
2015;(9):1153-1156,1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an appropriate and simple decellularization protocol for preparing decellularized liver bio-logical scaffold(DLBS) .Methods Through immune-ofluorescence analysis ,scanning electron microscope ,quantification of Glyco-samin-oglycans(GAGs) and DNA ,we evaluated the effectiveness of low concentrations of SDS(0 .25% ,0 .50% ) and 1% Triton X-100 in the preservation of extracellular matrix of rat livers .Then ,we assessed Cytotoxicity of DLBS treated with three methods by MTT assay .Results Residual DNA after SDS(0 .25% ,0 .50% ) treatments were below 50 ng/mg dry weight ,which were less than Triton X-100 treatments .The content of GAGs in the 0 .25% SDS and 1% Triton X-100-treated scaffolds were higher than other detergent-treated scaffolds .The result of MTT assay showed that the liver scaffolds treated with three methods were not cytotoxic to proliferation of C3A cells .Conclusion The protocol containing 0 .25% SDS and a perfusion rate of 5 mL/min could be a rapid and effective decellularization protocol .DLBS constructed using this protocol could be an ideal material for preparing a transplant-able organ .
