Hearing damage and DHA protection on cochlea cell after cisplatin-induced ototoxicity in guinea pigs
10.3969/j.issn.1671-8348.2015.09.006
- VernacularTitle:二十二碳六烯酸对豚鼠顺铂所致耳毒性的影响
- Author:
Yueqi WANG
;
Peiling CAI
- Publication Type:Journal Article
- Keywords:
cisplatin;
docosahexenoic acid(DHA);
ototoxicity
- From:
Chongqing Medicine
2015;(9):1171-1173,1176
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protection of DHA on the cisplatin cochlear injured modal animals ,and to explore the effect mechanism of DHA .Methods The animals were grouped randomly :control group ,DHA-treated group ,and cisplatin group .DHA-treated group at the doses of 500 mg?kg -1 ?d-1 .Control group and cisplatin group just treated vegetable oil at same doses ,all group′s treatment were lasting 30 day .Cisplatin intraperitoneal injection doses of 0 .25 mg/mL ,lasting 9 day ,begin with after DHA-treated 22 day .The intraperitoneal injections just do to DHA + cisplatin group and cisplatin group .Control group given in normal saline only .ABR was detected before the injection and after .Count the number of hair cells of cochlea after injection 9 day and detected the spiral ganglion of cochlea .Results After treatment ,ABR threshold of DHA-treated group was lower than that of the cisplatin group (P<0 .05) .DHA-treated group hair cell survival rate was significantly higher than that of cisplatin group(P<0 .05) .The spiral ganglion cells (SGCs) were severely damaged of cisplatin group than DHA-treated group .Conclusion DHA in-take may protect the cochlear from antioxidant injury of experiment animals ;these results may contribute to the treatments of cispl-atin caused deafness in clinic .
