Correlation of EGFR mutation with ERCC1 and TYMS mRNA expression in non-small cell lung cancer

VernacularTitle:非小细胞肺癌 EGFR基因突变与ERCC1、TYMS mRNA表达相关性的研究
Author: Quan ZHANG ; Qunyou TAN ; Ruwen WANG ; Shaolin TAO ; Poming KANG ; Bo DENG ; Jinghai ZHOU ; Kun LI ; Kai QIAN ; Bin JIANG
Publication Type:Journal Article
Keywords: non-small cell lung cancer; ERCC1; TYMS; EGFR
From: Chongqing Medicine 2015;(9):1177-1179,1183
CountryChina
Language:Chinese
Abstract: Objective To investigate whether EGFR gene mutations are correlated with the gene expression of ERCC1 and TYMS in non-small-cell lung cancer .Methods Collected February to December 2013 of non-small cell lung cancer(NSCLC) pa-tients eligible for enrolled 97 patients ,tumor tissue specimens obtained by intraoperative cut or puncture ,Gene expression of ERCC1 and TYMS were determined by branched-DNA liquid chip ,while somatic mutations in EGFR(E18 ,E19 ,E20 ,E21) gene were detec-ted by xTAG-liquid chip;And analysis of EGFR gene mutation associated with ERCC1 ,TYMS mRNA expression .Results Totally 29 cases of EGFR mutation were detected in all 97 specimens ,with a mutation rate of 30% (29/97) ,and a relatively high detection rate was observed in female ,adenocarcinoma and non-smoking patients(P<0 .05) .EGFR mutation was relevant to the expression of ERCC1(χ2 =4 .088 ,P<0 .05) ,EGFR mutation was irrelevant to the expression of TYMS(χ2 =0 .265 ,P>0 .05) .Conclusion In NSCLC tissues ,EGFR mutation is relevant to the expression of ERCC1 but irrelevant to the expression of TYMS .