Study on HPLC Characteristic Fingerprints of Lavandula Angustifolia
10.3969/j.issn.1005-5304.2015.04.024
- VernacularTitle:狭叶薰衣草特征指纹图谱研究
- Author:
Chenyang LI
;
Wei TAN
;
Yan CHEN
;
Jun ZHAO
;
Fang XU
- Publication Type:Journal Article
- Keywords:
Lavandula Angustifolia;
HPLC;
fingerprint;
rosmarinic acid;
principal composition cluster analysis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(4):87-90,91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprints of 20 batches of Lavandula Angustifolia by HPLC. Methods The determination was performed on a Phenomenex ODS-A column (250 mm× 4.6 mm, 5 μm). The mobile phase was in gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol/L phosphate acid solution. The flow rate was 1.0 mL/min. The temperature was 30 °C. The determine wavelength was 350 nm. The fingerprints of 20 batches of Lavandula Angustifolia were compared and classified by similarity evaluation, cluster analysis, and principal composition analysis. Results Totally 10 chromatographic peaks were extracted as the common peaks of Lavandula Angustifolia, and 2 peaks were identified. The similarity degrees of the 20 batches of Lavandula Angustifolia were above 0.9. All the batches of Lavandula Angustifolia were classified into 3 categories. Conclusion The method is simple and reproducible, and can be used for the standardization and quality control of Lavandula Angustifolia.
